Developing an environmental DNA protocol for Hazel Dormouse surveys

Recording Hazel Dormouse ( Muscardinus avellanarius ) presence allows conservation and mitigation efforts to be effectively directed towards populated sites. As it is a protected species, licences are required for survey methods which disturb the animals or their habitat. Therefore, reliable low disturbance techniques desirable. In this study, we tested potential of detecting environmental DNA (eDNA) from soil as method dormice. Firstly, designed species-specific primers associated PCR protocol using tissue samples Dormice. To ensure specificity, also checked against non-target species. Next, collected occupied sites in England test methodology with samples. This included testing site-level heterogeneity dormouse distribution localised level surrounding nest boxes. Finally, changes over time, after removal site. Using eDNA surveys promising. Our results indicate that usable can developed, providing relatively cost time-efficient tool when compared existing methods. Future work should aim further understand through time. Through identifying sites, could support projects help direct efforts.